Agrisera/HSP70 | Heat shock protein 70 (chloroplastic)/AS08 348/-免疫在线蚂蚁淘旗下平台-

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Agrisera/HSP70 | Heat shock protein 70 (chloroplastic)/AS08 348/

来自 : 发布时间：2024-12-23


product information
Background		Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
Immunogen		KLH-conjugated synthetic peptide derived from higher plant chloroplastic HSP70, inluding Arabidopsis thaliana cpHSC70-1, At4g24280 and cpHSC70-2, At5g49910
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		200 µl
Reconstitution		For reconstitution add 200 µl of sterile water.
Storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		western blot (WB), immunoprecipitation (IP)
Related products		AS08 371 \| anti-HSP70 \| heat shock protein 70 cytoplasmic AS08 347 \| anti-HSP70 \| heat shock protein 70, mitochondrial collection of antibodies to plant heat shock proteins
Additional information

application information
Recommended dilution		1: 2000 (WB), 1: 100 (IP)
Expected \| apparent MW		76 \| 70 kDa
Confirmed reactivity		Arabidopsis thaliana, Hordeum spontaneum, Oryza sativa, Pinus strobus, Pisum sativum
Predicted reactivity		Sorghum bicolor, Arundo donax, Brassica rapa subsp. pekinensis, Brassica napus, Coffea canephora, Theobroma cacao, Brachypodium distachyon, Prunus persica, Ricinus communis, Phaseolus vulgaris, Solanum tuberosum, Solanum lycopersicum, Citrus sinensis, Citrus clementina.dicots including: Spinacia oleracea, Vitis vinifera, Capsella rubella, Medicago trancatula, Glycine max, Glycine soja monocots including: Oryza sativa, Zea mays, Hordeum Vulgare, Triticum aestivum trees: Picea sitchemsis, Populus trichocarpa moss: Physomitrella patens
Not reactive in		no confirmed exceptions from predicted reactivity known in the moment
Additional information		To be added when available
Selected references		Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print] Jedmowski et al. (2014). Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley. Photosynthetica, September 2014.

application example

total protein from Arabidopsis thaliana chloroplasts (20 µg) and ,Arabidopsis thaliana leaf extracts (25 µg) were separated on 10% acrilamide gel and electrophoresis prepared according to Schägger and von Jagov (Anl. Biochem., 1987, 166:368-379). After running the gel, proteins were transferred to nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Transfer was checked by Ponceau S staining. Blot was destained by several quick washings in distilled water and 1 washing in 1X TBS (10 mM T pH 7.5, 150 mM NaCl) (10-15 min.).Blot was blocked by 1.5 hour in 5% milk in TBST (1X TBS, 0,1 20) After blocking blot was washed quickly twice in TBST and incubated 2 hours with primary antibody (dilution 1: 2000 TBST (dilution 1:1000). Washing: two quick washings in TBST and 3 x 10 min. washings in TBST. Then blot was incubated 45-60 min. with a secondary anti-rabbit antibodies conjugated to peroxidase (Sigma, dilution 1:10000) in TBST. Washing: as above. After washing blot was incubated 1-2 min. in ECL solution and exposed to Kodakautoradiography film. Exposure time was 10 seconds.

Courtesy Dr. J. Piechota

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发布于： 2024-12-23 阅读（）

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