Agrisera/UCP | Uncoupling protein/AS12 1850/-免疫在线蚂蚁淘旗下平台-

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Agrisera/UCP | Uncoupling protein/AS12 1850/

来自 : 发布时间：2024-12-23


product information
Background		UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2.
Immunogen		KLH-conjugated synthetic peptide derived from known UCP protein sequences, including UCP1 (AT3G54110) and UCP2 (AT5G58970) of Arabidopsis thaliana
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified serum
Format		Lyophilized in PBS pH 7.4
Quantity		200 µg
Reconstitution		For reconstitution add 200 µl of sterile water.
Storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		western blot (WB)
Related products		antibodies to other plant mitochondrial proteins Plant and algal protein extraction buffer Secondary antibodies
Additional information		Peptide used to elicit this antibody is conserved in both isoforms, UCP1 and UCP2 of Arabidopsis thaliana.

application information
Recommended dilution		1 : 2000 with standard ECL (WB)
Expected \| apparent MW		32 kDa
Confirmed reactivity		Arabidopsis thaliana, Splanum lycopersicum, Triticum aestivum, Vicia faba (protoplasts)
Predicted reactivity		Citrus sinensis, Dracunulus vulgaris, Glycine max, Litchi chinensis, Medicago tribuloides, Ricinus communis, Oryza sativa, Populus trichocarpa, Triticum aestivum, Saccharum officinarium (sugarcane),
Not reactive in		no confirmed exceptions from predicted reactivity are currently known
Additional information
Selected references		Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023. Florez-Sarasa et al. (2016). Impaired cyclic electron flow around Photosystem I disturbs high-light respiratory metabolism. Plant Physiol. 2016 Oct 19. pii: pp.01025.2016. Liu et al. (2015). Silencing of mitochondrial uncoupling protein gene aggravates chilling stress by altering mitochondrial respiration and electron transport in tomato. Acta Physiologiae Plantarum November 2015, 37:223. Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544. Grabelnych et al. (2014). Mitochondrial Energy Dissipating Systems (Alternative Oxidase, Uncoupling Proteins, and External NADH Dehydrogenase) Are Involved in Development of Frost Resistance of Winter Wheat Seedlings. ISSN 0006 2979, Biochemistry (Moscow), 2014, Vol. 79, No. 6, pp. 506 519. © Pleiades Publishing, Ltd., 2014.Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144.

application example

$\"western$

10 μg of mitochondrial fraction from Arabidopsis thaliana and 25 μg of Arabidopsis thaliana leaf extract were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 5000 anti-VDAC1 antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies from Agrisera, AS09 602 and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7).

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发布于： 2024-12-23 阅读（）

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